12-14 August 2015
Cuernavaca, Morelos
America/Mexico_City timezone
Home > Timetable > Session details > Contribution details

Contribution Poster

Cuernavaca, Morelos -

Effect of phytohormone 2-6-ᵧ-benzilaminopurine in the morphogenetic response of Laelia anceps Lindl. subsp. anceps (Orchidaceae)

Speakers

  • Mr. Luis CASTILLO PÉREZ

Primary authors

Co-authors

Abstract content

Laelia anceps Lindl. subsp. anceps is an endemic orchid of Mexico with a significant distribution in the Huasteca Potosina. In this region, anthropogenic activities such as fire forestakes, the change in land use and the illegal cutting of trees together with the removal of large numbers of plants for the illegal sale, this has caused serious damage in the number of wild orchid populations. A biotechnology alternative for the conservation of mexican orchids is the use of techniques such as Plant Tissue Culture as micropropagation. The aim was to analyze the effect of regulatory growth phytohormone 2-6-ᵧ-benzilaminopurine (BAP) in the morphogenetic plant response. The effect of BAP in the formation of new shoots and roots was evaluated. Were used as explants seedlings of three months old germinated in vitro in the laboratory of Environmental Sciences at the U.A.S.L.P. – U.A.M.Z.H. which they were cultured in a new medium supplemented with BAP (0, 2.5, 5 y 10 mg L-1) and coconut water (0.03 mL L-1). Oxidation and contamination rate and the number of shoots , the height , the number and length of roots was evaluated for three periods of 90 days each. A low rate of oxidation was showed. The formation of 14.5 ± 1 new bunds in the media with 10 mg L-1 BAP and 0.03 mL L-1 of coconut water was observed, treatment was also effective in stimulating the formation of the root system. The synchrotron light allows observation of inaccessible spaces for visible light, so that could be taken into account to study the organic chemical process and determine the exact location of this hormone in the plant tissue L. anceps Lindl. subsp. anceps and obtain high resolution images of different morphogenetic stages, also locating the interaction of BAP at the cellular level.